Ron Bouchard

Biography and Professional Achievements

Ron Bouchard comes to CCU with over 30 years of basic science research laboratory experience within the university and medical field. His expertise surrounds equipment and facilities management, systems operations, and the training of faculty, students, and staff on the use of sophisticated instrumentation and implementation of standard operating procedures. He is co-author on over 39 peer-reviewed scientific journals and publications.

Publications

• The Bcl-2 Homology-3 Domain (BH3)-Only Proteins, Bid, DP5/Hrk, and BNip3L, Are Upregulated in Reactive Astrocytes of End-Stage Mutant SOD1 Mouse Spinal Cord.
  Duval N, Sumner WA, Andrianakos AG, Gray JJ, Bouchard RJ, Wilkins HM, Linseman DA.
  Front Cell Neurosci. 2018 Jan 30;12:15. doi: 10.3389/fncel.2018.00015. eCollection 2018.
  PMID: 29440992
• Varicella zoster virus infection of human fetal lung cells alters mitochondrial morphology.
  Keller AC, Badani H, McClatchey PM, Baird NL, Bowlin JL, Bouchard R, Perng GC, Reusch JE, Kaufer BB, Gilden D, Shahzad A, Kennedy PG, Cohrs RJ.
  J Neurovirol. 2016 Oct;22(5):674-682. Epub 2016 May 31.
  PMID: 27245593
• Differential Mitochondrial Adaptation in Primary Vascular Smooth Muscle Cells from a Diabetic Rat Model.
  Keller AC, Knaub LA, McClatchey PM, Connon CA, Bouchard R, Miller MW, Geary KE, Walker LA, Klemm DJ, Reusch JE. Oxid Med Cell Longev. 2016;2016:8524267. doi: 10.1155/2016/8524267. Epub 2016 Jan 11.
  PMID: 27034743
• Raw and processed microscope images of fixed cells at baseline and following various experimental perturbations.
  McClatchey PM, Keller AC, Bouchard R, Knaub LA, Reusch JE.
  Data Brief. 2016 Jan 29;6:998-1006. doi: 10.1016/j.dib.2016.01.044. eCollection 2016 Mar.
  PMID: 26949731
• Fully automated software for quantitative measurements of mitochondrial morphology.
  McClatchey PM, Keller AC, Bouchard R, Knaub LA, Reusch JE.
  Mitochondrion. 2016 Jan;26:58-71. doi: 10.1016/j.mito.2015.12.003. Epub 2015
  Dec 11.PMID: 26688338
• Regulation of cyclic AMP response element-binding protein during neuroglial interactions.
  Qin L, Bouchard R, Pugazhenthi S. J Neurochem. 2016 Mar;136(5):918-30. doi: 10.1111/jnc.13497. Epub 2016 Jan 11. PMID: 26677139
• Corrigendum: depletion of tumor-associated macrophages slows the growth of chemically induced mouse lung adenocarcinomas.
  Fritz JM, Tennis MA, Orlicky DJ, Yin H, Ju C, Redente EF, Choo KS, Staab TA, Bouchard RJ, Merrick DT, Malkinson AM, Dwyer-Nield LD.Front Immunol. 2015 Mar 3;6:88. doi: 10.3389/fimmu.2015.00088. eCollection 2015.PMID: 25784911
• Dipeptidyl peptidase-4 inhibition in diabetic rats leads to activation of the transcription factor CREB in β-cells.
  Pugazhenthi S, Qin L, Bouchard R.Eur J Pharmacol. 2015 May 15;755:42-9. doi: 10.1016/j.ejphar.2015.02.024. Epub 2015 Feb 23.PMID: 25720341
• Neuronal apoptosis induced by selective inhibition of Rac GTPase versus global suppression of Rho family GTPases is mediated by alterations in distinct mitogen-activated protein kinase signaling cascades.
  Stankiewicz TR, Ramaswami SA, Bouchard RJ, Aktories K, Linseman DA.
  J Biol Chem. 2015 Apr 10;290(15):9363-76. doi: 10.1074/jbc.M114.575217. Epub 2015 Feb 9.PMID: 25666619
• Depletion of tumor-associated macrophages slows the growth of chemically induced mouse lung adenocarcinomas.
  Fritz JM, Tennis MA, Orlicky DJ, Lin H, Ju C, Redente EF, Choo KS, Staab TA, Bouchard RJ, Merrick DT, Malkinson AM, Dwyer-Nield LD. Front Immunol. 2014 Nov 25;5:587. doi: 10.3389/fimmu.2014.00587. eCollection 2014. Erratum in: Front Immunol. 2015;6:88.PMID: 25505466
• C-terminal binding proteins are essential pro-survival factors that undergo caspase-dependent downregulation during neuronal apoptosis.
  Stankiewicz TR, Schroeder EK, Kelsey NA, Bouchard RJ, Linseman DA.
  Mol Cell Neurosci. 2013 Sep;56:322-32. doi: 10.1016/j.mcn.2013.07.004. Epub 2013 Jul 13.PMID: 23859824
• Induction of an inflammatory loop by interleukin-1β and tumor necrosis factor-α involves NF-kB and STAT-1 in differentiated human neuroprogenitor cells.
  Pugazhenthi S, Zhang Y, Bouchard R, Mahaffey G.PLoS One. 2013 Jul 29;8(7):e69585. doi: 10.1371/journal.pone.0069585. Print 2013.PMID: 23922745
• N-terminal cleavage of the mitochondrial fusion GTPase OPA1 occurs via a caspase-independent mechanism in cerebellar granule neurons exposed to oxidative or nitrosative stress.
  Gray JJ, Zommer AE, Bouchard RJ, Duval N, Blackstone C, Linseman DA.
  Brain Res. 2013 Feb 4;1494:28-43. doi: 10.1016/j.brainres.2012.12.001. Epub 2012 Dec 7. PMID: 23220553
• Laser capture microdissection of neurons from differentiated human neuroprogenitor cells in culture.
  Bouchard R, Chong T, Pugazhenthi S.
  J Vis Exp. 2013 Sep 16;(79):e50487. doi: 10.3791/50487.
  PMID: 24084642
• Signal transducer and activator of transcription-5 mediates neuronal apoptosis induced by inhibition of Rac GTPase activity.
  Stankiewicz TR, Loucks FA, Schroeder EK, Nevalainen MT, Tyler KL, Aktories K, Bouchard RJ, Linseman DA. J Biol Chem. 2012 May 11;287(20):16835-48. doi: 10.1074/jbc.M111.302166. Epub 2012 Feb 29.PMID: 22378792
• Neuroprotective actions of glucagon-like peptide-1 in differentiated human neuroprogenitor cells.
  Velmurugan K, Bouchard R, Mahaffey G, Pugazhenthi S. J Neurochem. 2012 Dec;123(6):919-31. doi: 10.1111/jnc.12036. Epub 2012 Oct 25.PMID: 23020846
• Calpain plays a central role in 1-methyl-4-phenylpyridinium (MPP+)-induced neurotoxicity in cerebellar granule neurons.
  Harbison RA, Ryan KR, Wilkins HM, Schroeder EK, Loucks FA, Bouchard RJ, Linseman DA. Neurotox Res. 2011 Apr;19(3):374-88. Epub 2010 Mar 24.PMID:20333497
• CREB downregulation in vascular disease: a common response to cardiovascular risk.
  Schauer IE, Knaub LA, Lloyd M, Watson PA, Gliwa C, Lewis KE, Chait A, Klemm DJ, Gunter JM, Bouchard R, McDonald TO, O'Brien KD, Reusch JE. Arterioscler Thromb Vasc Biol. 2010 Apr;30(4):733-41. Epub 2010 Feb 11.PMID:20150559
• Caspases indirectly regulate cleavage of the mitochondrial fusion GTPase OPA1 in neurons undergoing apoptosis.
  Loucks FA, Schroeder EK, Zommer AE, Hilger S, Kelsey NA, Bouchard RJ, Blackstone C, Brewster JL, Linseman DA. Brain Res. 2009 Jan 23;1250:63-74. Epub 2008 Nov 18.PMID:19046944
• Green tea epigallocatechin 3-gallate accumulates in mitochondria and displays a selective antiapoptotic effect against inducers of mitochondrial oxidative stress in neurons.
  Schroeder EK, Kelsey NA, Doyle J, Breed E, Bouchard RJ, Loucks FA, Harbison RA, Linseman DA Antioxid Redox Signal. 2009 Mar;11(3):469-80.
• Dominant negative mutant forms of the cAMP response element binding protein induce apoptosis and decrease the anti-apoptotic action of growth factors in human islets.
  Sarkar SA, Gunter J, Bouchard R, Reusch JE, Wiseman A, Gill RG, Hutton JC, Pugazhenthi S.
  Diabetologia. 2007 Aug;50(8):1649-59. Epub 2007 Jun 26
• Glutathione binding to the Bcl-2 homology-3 domain groove: a molecular basis for Bcl-2 antioxidant function at mitochondria.
  Zimmermann AK, Loucks FA, Schroeder EK, Bouchard RJ, Tyler KL, Linseman DA.
  J Biol Chem. 2007 Oct 5;282(40):29296-304. Epub 2007 Aug 9. PMID: 17690097
• Tumor signaling to the bone marrow changes the phenotype of monocytes and pulmonary macrophages during urethane-induced primary lung tumorigenesis in A/J mice.
  Redente EF, Orlicky DJ, Bouchard RJ, Malkinson AM. Am J Pathol. 2007 Feb;170(2):693-708.PMID: 17255336
• Adhesion-related kinase induction of migration requires phosphatidylinositol-3-kinase and ras stimulation of rac activity in immortalized gonadotropin-releasing hormone neuronal cells.
  Nielsen-Preiss SM, Allen MP, Xu M, Linseman DA, Pawlowski JE, Bouchard RJ, Varnum BC, Heidenreich KA, Wierman ME. Endocrinology. 2007 Jun;148(6):2806-14. Epub 2007 Mar 1. PMID: 17332061
• Endoplasmic reticulum stress and trophic factor withdrawal activate distinct signaling cascades that induce glycogen synthase kinase-3 beta and a caspase-9-dependent apoptosis in cerebellar granule neurons.
  Brewster JL, Linseman DA, Bouchard RJ, Loucks FA, Precht TA, Esch EA, Heidenreich KA.
  Mol Cell Neurosci. 2006 Jul;32(3):242-53. PMID: 16765055
• Proteasome inhibition elicits a biphasic effect on neuronal apoptosis via differential regulation of pro-survival and pro-apoptotic transcription factors.
  Butts BD, Hudson HR, Linseman DA, Le SS, Ryan KR, Bouchard RJ, Heidenreich KA. Mol Cell Neurosci. 2005 Oct;30(2):279-89. PMID: 16112871
• Inhibition of Rac GTPase triggers a c-Jun- and Bim-dependent mitochondrial apoptotic cascade in cerebellar granule neurons.
  Le SS, Loucks FA, Udo H, Richardson-Burns S, Phelps RA, Bouchard RJ, Barth H, Aktories K, Tyler KL, Kandel ER, Heidenreich KA, Linseman DA. J Neurochem. 2005 Aug;94(4):1025-39. PMID: 16092944
• Abstract, Distinct mechanisms of neuronal apoptosis are triggered by antagonism of Bcl-2/Bcl-x(L) versus induction of the BH3-only protein Bim.
  Zimmermann AK, Loucks FA, Le SS, Butts BD, Florez-McClure ML, Bouchard RJ, Heidenreich KA, Linseman DA.J Neurochem. 2005 Jul;94(1):22-36. PMID: 15953346
• The permeability transition pore triggers Bax translocation to mitochondria during neuronal apoptosis. Precht TA, Phelps RA, Linseman DA, Butts BD, Le SS, Laessig TA, Bouchard RJ, Heidenreich KA.
  Cell Death Differ. 2005 Mar;12(3):255-65. PMID: 15637643
• Glycogen synthase kinase-3beta phosphorylates Bax and promotes its mitochondrial localization during neuronal apoptosis.
  Linseman DA, Butts BD, Precht TA, Phelps RA, Le SS, Laessig TA, Bouchard RJ, Florez-McClure ML, Heidenreich KA. J Neurosci. 2004 Nov 3;24(44):9993-10002. PMID: 15525785
• Novel nuclear herniations induced by nuclear localization of a viral protein.
  Hoyt CC, Bouchard RJ, Tyler KL. J Virol. 2004 Jun;78(12):6360-9. PMID: 15163729
• The p75 neurotrophin receptor can induce autophagy and death of cerebellar Purkinje neurons.
  Florez-McClure ML, Linseman DA, Chu CT, Barker PA, Bouchard RJ, Le SS, Laessig TA, Heidenreich KA.
  J Neurosci. 2004 May 12;24(19):4498-509. PMID: 15140920
• Caspase inhibition protects against reovirus-induced myocardial injury in vitro and in vivo.
  DeBiasi RL, Robinson BA, Sherry B, Bouchard R, Brown RD, Rizeq M, Long C, Tyler KL.
  J Virol. 2004 Oct;78(20):11040-50.
  PMID: 15452224
• Cytokine-mediated down-regulation of the transcription factor cAMP-response element-binding protein in pancreatic beta-cells.
  Jambal P, Masterson S, Nesterova A, Bouchard R, Bergman B, Hutton JC, Boxer LM, Reusch JE, Pugazhenthi S. J Biol Chem. 2003 Jun 20;278(25):23055-65. Epub 2003 Apr 5. PMID:12679364
• The pesticide rotenone induces caspase-3-mediated apoptosis in ventral mesencephalic dopaminergic neurons.
  Ahmadi FA, Linseman DA, Grammatopoulos TN, Jones SM, Bouchard RJ, Freed CR, Heidenreich KA, Zawada WM. J Neurochem. 2003 Nov;87(4):914-21. PMID: 14622122
• Inactivation of the myocyte enhancer factor-2 repressor histone deacetylase-5 by endogenous Ca(2+) //calmodulin-dependent kinase II promotes depolarization-mediated cerebellar granule neuron survival. Linseman DA, Bartley CM, Le SS, Laessig TA, Bouchard RJ, Meintzer MK, Li M, Heidenreich KA. J Biol Chem. 2003 Oct 17;278(42):41472-81. Epub 2003 Aug 1. PMID: 12896970
• A myocyte enhancer factor 2D (MEF2D) kinase activated during neuronal apoptosis is a novel target inhibited by lithium.
  Linseman DA, Cornejo BJ, Le SS, Meintzer MK, Laessig TA, Bouchard RJ, Heidenreich KA. J Neurochem. 2003 Jun;85(6):1488-99. PMID: 12787068
• Insulin-like growth factor-I blocks Bcl-2 interacting mediator of cell death (Bim) induction and intrinsic death signaling in cerebellar granule neurons.
  Linseman DA, Phelps RA, Bouchard RJ, Le SS, Laessig TA, McClure ML, Heidenreich KA. J Neurosci. 2002 Nov 1;22(21):9287-97. PMID: 12417654
• Suppression of death receptor signaling in cerebellar Purkinje neurons protects neighboring granule neurons from apoptosis via an insulin-like growth factor I-dependent mechanism.
  Linseman DA, cClure ML, Bouchard RJ, Laessig TA, Ahmadi FA, Heidenreich KA. J Biol Chem. 2002 Jul 5;277(27):24546-53. Epub 2002 Apr 18.PMID: 11964396